Used primarily in the synthesis of pharmaceutical compounds, particularly as an intermediate in the production of antifolate agents and antimicrobial drugs. It serves as a building block in the development of kinase inhibitors and other biologically active molecules targeting cancer and inflammatory diseases. Its structure supports binding to enzyme active sites, making it valuable in medicinal chemistry for designing inhibitors that disrupt specific metabolic pathways in pathogens or abnormal cells. Also employed in research settings to develop novel pyrimidine-based therapeutics with improved selectivity and efficacy.