5-Methyl-6-oxo-1,6-dihydropyridine-2-carboxylic acid is primarily utilized in the field of pharmaceutical research and development. It serves as a key intermediate in the synthesis of various bioactive compounds and drug candidates. Its structure is particularly valuable in the creation of molecules with potential therapeutic applications, such as anti-inflammatory, antimicrobial, or antiviral agents. Additionally, it is employed in organic synthesis to construct complex heterocyclic frameworks, which are often found in many biologically active substances. Researchers also explore its use in the development of novel enzyme inhibitors, leveraging its unique chemical properties to target specific biological pathways.