2-Amino-5-nitronicotinic acid is primarily used in the field of organic synthesis as a building block for the preparation of more complex chemical compounds. It serves as an intermediate in the synthesis of various pharmaceutical agents, particularly those targeting specific enzymes or receptors. Additionally, it is utilized in the development of dyes and pigments due to its nitro and amino functional groups, which contribute to color formation. In research, this compound is often employed in the study of heterocyclic chemistry, helping to explore new reactions and pathways for creating novel molecules with potential biological or industrial applications.