3-Aminoisonicotinohydrazide is primarily utilized in the field of medicinal chemistry and pharmaceutical research. It serves as a key intermediate in the synthesis of various biologically active compounds, particularly those with potential therapeutic applications. Its structure is often exploited in the development of antimicrobial, antifungal, and antitumor agents due to its ability to interact with biological targets effectively. Additionally, it is employed in the design of hydrazide-based ligands for coordination chemistry, which can be used to create metal complexes with potential catalytic or medicinal properties. Researchers also explore its role in the synthesis of heterocyclic compounds, which are important in drug discovery and development.