N-(tert-Butyl)isonicotinamide is primarily utilized in the field of medicinal chemistry as an intermediate in the synthesis of more complex pharmaceutical compounds. Its structure makes it a valuable building block for developing molecules with potential therapeutic applications, particularly in the design of drugs targeting neurological disorders and infectious diseases. Researchers also explore its use in creating ligands for biological receptors, aiding in the study of biochemical pathways and drug discovery processes. Additionally, it has been investigated for its role in modulating enzyme activity, which could lead to the development of novel enzyme inhibitors for treating various medical conditions.