1-Pyrenebutyric acid is widely used in fluorescence studies due to its strong fluorescent properties. It serves as a fluorescent probe in biochemical and biophysical research to study molecular interactions, membrane dynamics, and protein binding.

In material science, it is utilized to modify surfaces and create functionalized materials with enhanced optical properties. It is also employed in the synthesis of organic semiconductors and conjugated polymers, where its fluorescence aids in characterizing electronic structures.

Additionally, 1-Pyrenebutyric acid is used in environmental studies to track pollutants and analyze their behavior in various systems. Its ability to bind with other molecules makes it valuable in sensor development for detecting specific analytes.

In nanotechnology, it is incorporated into nanostructures to improve their luminescent characteristics, making it useful in imaging and diagnostic applications. Its versatility in research and industrial applications underscores its importance in advancing scientific and technological innovations.