1-Aminopyrene is widely utilized as a fluorescent probe in biochemical and environmental studies due to its strong fluorescence properties. It is commonly employed in the detection and analysis of DNA adducts, helping researchers understand the interactions between DNA and various chemical agents, which is crucial in cancer research and toxicology. Additionally, it is used in the study of polycyclic aromatic hydrocarbons (PAHs) to assess environmental pollution and monitor the presence of these harmful compounds in air, water, and soil samples. Its ability to bind to proteins and lipids also makes it valuable in cellular imaging and the investigation of molecular dynamics within biological systems.