1,3-Diaminopyrene is primarily used as a fluorescent dye in various scientific and industrial applications. Its strong fluorescence properties make it valuable in biological staining, where it helps in visualizing cellular structures and processes under a microscope. In material science, it is utilized in the development of organic light-emitting diodes (OLEDs) and other optoelectronic devices due to its ability to emit light efficiently. Additionally, it serves as a probe in environmental studies to detect and analyze pollutants, particularly polycyclic aromatic hydrocarbons (PAHs), by binding to them and emitting detectable signals. Its application extends to chemical sensing, where it aids in the identification and quantification of specific molecules in complex mixtures.