This compound is primarily utilized in biochemical research and pharmaceutical development due to its role as a pteridine derivative. It is often employed in studies related to enzyme inhibition, particularly targeting enzymes involved in folate metabolism. Its structural properties make it a valuable tool for investigating metabolic pathways and designing potential therapeutic agents. Additionally, it may be used in the synthesis of analogs for drug discovery, especially in areas like cancer research and antimicrobial development. Its phosphate form enhances solubility, making it more suitable for experimental applications in aqueous environments.