Used as an intermediate in the synthesis of biologically active compounds, particularly in the development of antifolate agents. Plays a role in research related to enzyme inhibitors, especially in studies targeting dihydrofolate reductase (DHFR). Also utilized in the preparation of pteridine-based derivatives for pharmaceutical and biochemical applications, including potential use in anticancer and antimicrobial drug research. Its structure supports conjugation in probe development for cellular imaging and diagnostic tools.