This compound is primarily utilized in the field of medicinal chemistry and biochemistry, particularly in the development of enzyme inhibitors. It serves as a key intermediate in the synthesis of antifolate agents, which are crucial in targeting folate-dependent enzymes. These inhibitors are often employed in cancer therapy to disrupt the growth of malignant cells by interfering with DNA synthesis and repair. Additionally, it finds application in research focused on understanding the mechanisms of folate metabolism and related pathways, aiding in the development of treatments for various diseases, including bacterial infections and autoimmune disorders. Its structural features make it valuable for designing molecules with enhanced specificity and potency in therapeutic applications.