Used in research and diagnostic applications to study hemoglobinopathies such as beta-thalassemia, where elevated levels of hemoglobin A2 are a key clinical marker. The ferrous-stabilized form maintains the iron in the reduced (Fe²⁺) state, preserving the protein’s functional conformation for accurate analytical measurements. It serves as a reference standard in HPLC and electrophoresis techniques for hemoglobin variant analysis. Also utilized in the development and calibration of clinical assays for blood disorder screening and in pharmaceutical research for oxygen carrier studies.