REV7/REV3L-IN-1 is primarily used in research settings to study DNA damage and repair mechanisms. It acts as an inhibitor of the REV7-REV3L interaction, which is crucial for the translesion synthesis (TLS) pathway. By blocking this interaction, it helps researchers understand how cells bypass DNA lesions and the role of TLS in maintaining genomic stability. This chemical is particularly valuable in cancer research, as targeting the TLS pathway could potentially enhance the efficacy of DNA-damaging chemotherapy agents. Additionally, it aids in exploring the mechanisms of drug resistance in cancer cells, providing insights for developing novel therapeutic strategies.