Used primarily in biochemical assays, it serves as a key component in the Bicinchoninic Acid (BCA) protein assay. This method is widely employed for quantifying protein concentration due to its high sensitivity and compatibility with various substances. The compound reacts with proteins in the presence of copper ions, producing a purple-colored complex. The intensity of this color, measured spectrophotometrically, correlates directly with protein concentration. It is particularly valued for its ability to detect low protein levels in complex biological samples, making it essential in research, diagnostics, and quality control in biotechnology and pharmaceutical industries. Its stability and ease of use further enhance its application in high-throughput screening and routine laboratory analyses.