tert-Butyl (2-(2-acetylhydrazinyl)-2-oxoethyl)carbamate is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the development of various bioactive compounds, particularly in the synthesis of hydrazide derivatives. These derivatives are often explored for their potential therapeutic applications, such as antimicrobial, anticancer, and anti-inflammatory agents. Additionally, the compound is utilized in peptide chemistry for protecting groups during the synthesis of complex peptides, ensuring selective reactions and stability of the intermediates. Its role in medicinal chemistry is significant, as it contributes to the design and optimization of drug candidates with improved efficacy and safety profiles.