Widely used in oligonucleotide synthesis, this compound serves as a key protecting group reagent for the 5′-hydroxyl group of nucleosides. Its primary role is in the solid-phase synthesis of DNA and RNA, where it ensures selective protection during phosphoramidite-based coupling steps. The trityl group provides colorimetric monitoring of deprotection due to the bright orange trityl cation released, allowing real-time assessment of coupling efficiency. The tert-butyldiphenylsilyl (TBDPS) component adds stability under various reaction conditions, making it suitable for multi-step syntheses of complex nucleic acid sequences. It is especially valuable in the production of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids where high purity and yield are critical.