Amyl 2-acetamido-2-deoxy-β-D-glucopyranoside is primarily used in biochemical and glycobiology research. It serves as a substrate or intermediate in the study of glycosylation processes and enzyme mechanisms, particularly those involving glycosidases and glycosyltransferases. This compound is valuable for understanding carbohydrate metabolism and the role of glycoconjugates in cellular functions. It is also utilized in the synthesis of complex carbohydrates and glycoproteins, aiding in the development of therapeutic agents and vaccines. Additionally, it finds application in the investigation of cell signaling pathways and the structural analysis of glycans. Its role in mimicking natural glycosides makes it a useful tool for probing biological interactions and designing inhibitors for carbohydrate-processing enzymes.