Z-Gly-Phe-OH is primarily used in peptide synthesis as a building block for constructing larger peptide chains. It serves as a protected dipeptide, where the Z-group (benzyloxycarbonyl) acts as a protective group for the amino terminus, preventing unwanted reactions during the synthesis process. This compound is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase peptide synthesis, enabling the controlled assembly of peptides with specific sequences. Its application is significant in the development of pharmaceuticals, bioactive peptides, and research tools for studying peptide structure and function. Additionally, it is employed in the production of peptide-based drugs, enzyme inhibitors, and other therapeutic agents.