Fmoc-glu(otbu)-thr(psi(Me,Me)pro)-OH is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a building block for constructing complex peptide chains, where the Fmoc group acts as a protective group for the amino acid during the synthesis process. The compound is especially valuable in the synthesis of peptides containing pseudoproline dipeptides, which help reduce aggregation and improve solubility during peptide chain assembly. This makes it a crucial tool in the development of therapeutic peptides, peptide-based drugs, and biochemical research applications. Its use enhances the efficiency and yield of peptide synthesis, particularly for challenging sequences.