N-Acetyl-D-proline is primarily utilized in the field of peptide synthesis and pharmaceutical research. It serves as a building block for the creation of complex peptides and proteins, aiding in the study of their structure and function. This compound is particularly valuable in the development of peptide-based drugs, where it helps improve stability and bioavailability. Additionally, it is employed in biochemical studies to investigate enzyme mechanisms and receptor interactions, contributing to the advancement of therapeutic strategies. Its role in chiral synthesis also makes it useful in producing enantiomerically pure compounds, which are critical in drug development and fine chemical manufacturing.