N-Acetyl-L-Isoleucine is primarily used in the field of biochemistry and nutrition as a dietary supplement. It is valued for its role in enhancing muscle recovery and growth, making it popular among athletes and bodybuilders. The compound is also utilized in research studies to investigate amino acid metabolism and protein synthesis. Additionally, it finds application in the formulation of specialized nutritional products aimed at supporting overall health and wellness. In the cosmetic industry, it is incorporated into skincare products for its potential to improve skin hydration and elasticity.