Fmoc-D-Allo-Ile-OH is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a building block for incorporating D-allo-isoleucine into peptide chains, enabling the creation of peptides with specific stereochemistry. This compound is valuable in research and drug development, where precise control over peptide structure is essential. Its Fmoc (fluorenylmethyloxycarbonyl) protecting group allows for selective deprotection during synthesis, ensuring efficient and controlled peptide assembly. It is commonly applied in the study of peptide-protein interactions, enzyme inhibitors, and the development of therapeutic peptides.