This compound is widely used in peptide synthesis as a protected form of hydroxyproline. It serves as a key intermediate in the production of peptides and peptidomimetics, particularly in the synthesis of collagen-like peptides. The Boc (tert-butoxycarbonyl) group provides protection for the amine functionality during peptide coupling reactions, while the methyl ester protects the carboxyl group. Its hydroxyl group can be further modified or used to introduce additional functional groups. The compound is also employed in the preparation of proline-based chiral catalysts and ligands for asymmetric synthesis. In medicinal chemistry, it is utilized to develop proline-containing drug candidates, especially for targeting collagen-related disorders or as building blocks for bioactive molecules. Its stability and ease of deprotection make it a valuable tool in organic synthesis and pharmaceutical research.