This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amino terminus, while the OtBu (tert-butyl) group protects the side chain carboxyl group of aspartic acid. The Hmb (2-hydroxy-4-methoxybenzyl) group is incorporated into the glycine residue to prevent aspartimide formation, a common side reaction during peptide synthesis. This compound is especially valuable in the synthesis of complex peptides or those containing aspartic acid, ensuring higher purity and yield by minimizing unwanted side reactions. Its application is critical in the production of peptides for pharmaceutical research, drug development, and biochemical studies.