Used extensively in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, ensuring specific amino acids are incorporated correctly during peptide chain assembly. The tert-butoxy and sulfonyl groups provide stability and prevent unwanted side reactions during synthesis. The fluorenylmethoxycarbonyl (Fmoc) group is crucial for temporary protection of the amino group, allowing for selective deprotection and elongation of the peptide chain. This compound is especially valuable in the synthesis of complex peptides and proteins, enabling researchers to produce high-purity peptides for pharmaceutical, biochemical, and biotechnological applications. Its unique structure also makes it suitable for synthesizing peptides with modified or non-natural amino acids, expanding its utility in drug development and research.