Boc-β-HoPhe-OH is primarily used in peptide synthesis as a protected amino acid derivative. The Boc (tert-butoxycarbonyl) group serves as a protective group for the amine functionality, preventing unwanted reactions during the peptide chain assembly process. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it ensures selective deprotection and coupling steps. Its application extends to the preparation of complex peptides and peptidomimetics, which are essential in drug discovery and development. Additionally, it is employed in the synthesis of bioactive peptides and modified amino acids, contributing to the study of structure-activity relationships in pharmaceutical research.