N-Carbobenzoxy-D-tyrosine is widely used in peptide synthesis as a protecting group for the amino acid tyrosine. Its primary application lies in preventing unwanted reactions at the tyrosine residue during the stepwise construction of peptide chains. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps maintain the integrity of the peptide sequence by selectively blocking the tyrosine side chain. After the peptide synthesis is complete, the carbobenzoxy group can be removed under mild conditions, allowing for the final product to retain its functional properties. Additionally, it is employed in the study of enzyme-substrate interactions and in the development of peptide-based pharmaceuticals, where precise control over amino acid reactivity is crucial.