This chemical is primarily used in peptide synthesis, where it serves as a protected form of L-isoleucine. The carbobenzoxy (Cbz) group acts as a protecting agent for the amino group, preventing unwanted reactions during the synthesis process. The dicyclohexylammonium salt form enhances its solubility in organic solvents, making it easier to handle in various synthetic procedures. It is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase peptide synthesis, ensuring the controlled and efficient incorporation of isoleucine into peptide chains. Additionally, it finds applications in the preparation of modified peptides and bioactive compounds for pharmaceutical research and development.