N²-[(9H-Fluoren-9-ylmethoxy)carbonyl]-2-methyl-L-asparagine is an Fmoc-protected derivative of the non-standard amino acid 2-methyl-L-asparagine, used as a building block in peptide synthesis. The Fmoc group protects the α-amino group, enabling selective reactions during peptide bond formation in solid-phase peptide synthesis (SPPS). It prevents unwanted side reactions, maintains amino acid integrity, and the 2-methyl substitution improves the stability and efficiency of synthesized peptides. Commonly applied in pharmaceutical and biochemical research for producing complex peptides and proteins, its acidic stability facilitates deprotection in synthesis workflows.