N-Acetyl-DL-valine is primarily used in the field of biochemistry and pharmaceutical research. It serves as a building block in the synthesis of peptides and proteins, aiding in the study of their structure and function. This compound is also utilized in the development of drug formulations, particularly in enhancing the stability and bioavailability of active pharmaceutical ingredients. Additionally, it finds application in the production of chiral compounds, where it acts as a resolving agent to separate enantiomers, which is crucial in creating more effective and targeted medications. In industrial settings, N-Acetyl-DL-valine is employed in the manufacture of specialty chemicals and as an intermediate in organic synthesis processes. Its role in these areas underscores its importance in advancing both scientific research and practical applications in chemistry and medicine.