N-Boc-2-bromo-D-phenylalanine is primarily used in the synthesis of peptides and pharmaceutical compounds. It serves as a key intermediate in the production of biologically active molecules, particularly in the development of protease inhibitors and other therapeutic agents. The Boc (tert-butoxycarbonyl) protecting group ensures selective reactivity during peptide coupling reactions, while the bromine atom allows for further functionalization through cross-coupling reactions. Its application is significant in medicinal chemistry for designing and optimizing drug candidates with enhanced specificity and efficacy. Additionally, it is utilized in research to study enzyme mechanisms and protein interactions, contributing to advancements in drug discovery and biochemical studies.