L-N-Boc-5-chlorotryptophan is primarily used in peptide synthesis and pharmaceutical research. It serves as a protected intermediate for the incorporation of tryptophan derivatives into peptides, ensuring selective reactions during synthesis. The Boc group provides stability and prevents unwanted side reactions, while the chloro substitution enhances the compound's reactivity in specific chemical processes. This chemical is particularly valuable in the development of bioactive peptides and drug candidates, where precise structural modifications are required. Its application extends to studying enzyme interactions and designing inhibitors for therapeutic purposes.