2-((tert-Butoxycarbonyl)amino)malonic acid is primarily used in organic synthesis as a building block for the preparation of more complex molecules. It serves as a key intermediate in the synthesis of peptides and other biologically active compounds. The tert-butoxycarbonyl (Boc) group in the molecule acts as a protective group for amines, allowing selective reactions to occur without affecting the amino functionality. This compound is particularly useful in the development of pharmaceuticals, where it aids in the controlled assembly of amino acids and other organic structures. Additionally, it is employed in the production of fine chemicals and research materials, where its reactivity and protective group properties are leveraged to achieve precise chemical transformations.