This chemical is primarily used in peptide synthesis as a protected form of threonine. The tert-butyl (tBu) group protects the hydroxyl side chain, while the Z-group (benzyloxycarbonyl) protects the amino group, ensuring selective reactions during peptide bond formation. The dicyclohexylamine (DCHA) salt form enhances solubility and stability, making it easier to handle in organic solvents. It is particularly valuable in solid-phase peptide synthesis (SPPS) and solution-phase synthesis, where precise control over amino acid reactivity is required. Its application is crucial in producing peptides for pharmaceutical research, drug development, and biochemical studies.