N-Boc-trans-4-fluoro-L-proline methyl ester is widely used in the synthesis of peptides and peptidomimetics, where it serves as a key building block. Its incorporation into peptide chains enhances stability and can influence the conformational properties of the resulting peptides. This compound is particularly valuable in medicinal chemistry for the development of drug candidates, as the fluorine atom can improve bioavailability and metabolic stability. It is also utilized in the preparation of proline derivatives for studying enzyme inhibition and receptor binding, making it a crucial component in biochemical research and pharmaceutical development.