This compound is primarily used in peptide synthesis and research applications. It serves as a protected form of proline, a crucial amino acid in the construction of peptides and proteins. The ethyl ester group provides stability during the synthesis process, while the HCl salt form enhances its solubility in various solvents. It is particularly valuable in solid-phase peptide synthesis (SPPS) for creating specific peptide sequences with high precision. Additionally, it finds use in studying peptide structure-activity relationships and in the development of pharmaceuticals, especially those targeting enzymatic or receptor-based mechanisms. Its role in producing bioactive peptides makes it essential in drug discovery and biochemical research.