D-Norleucine methyl ester hydrochloride is primarily used in peptide synthesis as a building block for creating specific peptide sequences. It is valuable in research and development for modifying peptide structures to study their biological activity and stability. This compound is also employed in the pharmaceutical industry to develop peptide-based drugs, particularly for targeting specific diseases or improving drug delivery systems. Additionally, it serves as a reagent in organic chemistry for synthesizing complex molecules with potential therapeutic applications. Its role in enhancing the understanding of peptide interactions makes it a crucial tool in biochemistry and medicinal chemistry.