This chemical is primarily used in peptide synthesis as a protected amino acid derivative. The tert-butoxy group provides steric protection, while the fluoren-9-ylmethoxycarbonyl (Fmoc) group acts as a protecting group for the amine functionality during peptide coupling reactions. It is particularly valuable in solid-phase peptide synthesis (SPPS), where selective deprotection of the Fmoc group allows for sequential addition of amino acids to build peptide chains. Its stereochemistry (2S,4S) ensures the correct chiral configuration in the final peptide product, making it essential for synthesizing biologically active peptides with high precision. Additionally, its stability under basic conditions and ease of removal of the Fmoc group make it a preferred choice in pharmaceutical research and development.