N-Formyl-DL-phenylalanine is primarily used in biochemical research and pharmaceutical development. It serves as a key intermediate in the synthesis of peptides and other complex organic compounds. Researchers utilize it to study enzyme mechanisms, particularly those involving formyl group transfer. In drug development, it acts as a precursor for designing molecules with potential therapeutic effects, such as anti-inflammatory or antimicrobial agents. Additionally, it is employed in the production of chiral compounds, aiding in the study of stereochemistry and its impact on biological activity. Its role in peptide synthesis makes it valuable for creating analogs that mimic natural peptides for further biological evaluation.