N-Formyl-DL-alanine is primarily used in biochemical research and peptide synthesis. It serves as a building block in the preparation of modified peptides and proteins, aiding in the study of their structure and function. This compound is also utilized in the development of enzyme inhibitors, which are crucial for understanding enzymatic pathways and designing therapeutic agents. Additionally, it finds application in the field of microbiology, where it is used to investigate bacterial chemotaxis and other cellular processes. Its role in these areas makes it a valuable tool in both academic and industrial research settings.