N-Formyl-D-phenylalanine is primarily utilized in peptide synthesis and biochemical research. It serves as a protected form of phenylalanine, where the formyl group acts as a protective moiety for the amino group during peptide chain assembly. This protection is crucial to prevent unwanted side reactions and ensure the correct sequence of amino acids in the final peptide product. Additionally, it is used in studies involving enzyme specificity and substrate interactions, particularly in the context of formyl peptide receptors, which play a role in immune responses. Its application extends to the development of peptide-based drugs and therapeutic agents, where precise control over amino acid incorporation is essential.