(S)-3-((tert-Butoxycarbonyl)amino)hex-5-enoic acid is primarily used in the synthesis of complex organic molecules, particularly in the field of medicinal chemistry. It serves as a key intermediate in the production of peptide-based drugs, where its structure allows for the incorporation of specific amino acid sequences. The compound is also utilized in the development of enzyme inhibitors, leveraging its chiral center to enhance selectivity and efficacy. Additionally, it finds application in the preparation of bioactive compounds for research purposes, aiding in the study of biochemical pathways and drug discovery processes. Its unique combination of functional groups makes it a versatile building block in organic synthesis.