This compound is primarily used in peptide synthesis as a protected form of serine. The tert-butyl group protects the hydroxyl side chain, while the carbobenzoxy (Cbz) group safeguards the amine functionality. This dual protection allows for selective deprotection during the synthesis of complex peptides, ensuring the integrity of the serine residue. It is particularly valuable in the production of pharmaceuticals and bioactive peptides where precise amino acid sequences are critical. Additionally, its methyl ester form enhances solubility in organic solvents, facilitating reactions in non-aqueous environments.