This compound is primarily utilized in the synthesis of peptides and pharmaceutical intermediates. It serves as a protected form of threonine, ensuring the amino acid's side chain remains stable during complex chemical reactions. Its tert-butyl ester group provides steric protection, preventing unwanted interactions during peptide bond formation. This makes it valuable in the production of custom peptides for research, drug development, and biotechnology applications. Additionally, its acetate salt form enhances solubility, facilitating its use in aqueous reaction environments. The compound is particularly useful in solid-phase peptide synthesis, where selective deprotection is required to achieve high-purity peptide products.