O-benzyl-threonine is widely used in peptide synthesis as a protected form of threonine. The benzyl group acts as a protective group for the hydroxyl side chain, preventing unwanted reactions during the peptide assembly process. This protection is crucial in solid-phase peptide synthesis (SPPS), where selective deprotection is required to achieve the desired peptide sequence. Additionally, it is utilized in the development of pharmaceutical compounds, particularly in the synthesis of bioactive peptides and peptidomimetics. Its application extends to research in biochemistry and medicinal chemistry, where it serves as a building block for designing and studying modified peptides with potential therapeutic properties.