This chemical is primarily used in peptide synthesis as a protecting group for amino acids. The fluorenylmethoxycarbonyl (Fmoc) group protects the amine functionality during the synthesis process, allowing selective deprotection and coupling of amino acids. The benzyl group protects the hydroxyl group of threonine, preventing unwanted reactions. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it ensures the precise assembly of peptide chains. Its application is critical in the production of peptides for pharmaceutical research, drug development, and biochemical studies. The compound’s stability and ease of removal under mild conditions make it a preferred choice in modern peptide chemistry.