This chemical is primarily used in peptide synthesis as a protected amino acid derivative. It serves as a building block for constructing peptides, particularly in solid-phase peptide synthesis (SPPS). The fluorenylmethoxycarbonyl (Fmoc) group protects the amino group during the synthesis process, ensuring selective reactions at other sites. The tert-butoxybenzyl group provides additional stability to the molecule under acidic conditions, making it suitable for complex peptide sequences. Its application is crucial in pharmaceutical research for developing peptide-based drugs, studying protein interactions, and exploring biochemical pathways. The compound’s design allows for efficient deprotection and coupling steps, enhancing the overall yield and purity of synthesized peptides.