This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected form of β-hydroxyproline, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a protecting group for the amino functionality. This allows for the controlled and sequential addition of amino acids during peptide chain assembly. The β-hydroxyproline moiety is significant in the synthesis of peptides that mimic collagen-like structures or other biologically active peptides. Its application is crucial in the development of therapeutic peptides, research peptides, and in the study of protein structure and function. The Fmoc protection ensures that the amino group remains inert during the coupling reactions, and it can be selectively removed under mild basic conditions, making it compatible with a wide range of peptide synthesis protocols.