Fmoc-N-(butyl)-glycine is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. Its primary application is as a building block for introducing butyl-glycine residues into peptide chains. The Fmoc (fluorenylmethyloxycarbonyl) protecting group ensures selective deprotection during synthesis, allowing for precise control over peptide assembly. This compound is especially valuable in creating peptides with modified backbones or side chains, which are often used in drug development, biochemical research, and the study of protein interactions. Its incorporation into peptides can enhance stability, alter hydrophobicity, or influence conformational properties, making it a versatile tool in designing bioactive molecules. Additionally, it is utilized in the synthesis of peptidomimetics, which mimic natural peptides for therapeutic or investigative purposes.