H-Gly-NHMe HCl is the hydrochloride salt of glycine N-methylamide, a derivative of glycine with the carboxylic acid group converted to an N-methylamide. This modification protects the C-terminus, making it suitable as a building block in peptide synthesis, particularly for incorporating N-methylamide-capped peptides. The free N-terminal amine allows for coupling to the C-terminus of peptide fragments or activated acids, commonly in solution-phase synthesis or for C-terminal extension in custom peptide assemblies. It is essential in solid-phase peptide synthesis (SPPS) variants or fragment condensation strategies to achieve specific C-terminal modifications that improve peptide stability, bioavailability, or receptor interactions. Furthermore, it supports biochemical research on enzyme substrates and peptide therapeutics, and in medicinal chemistry for developing novel peptide-based drugs.